Immunohistochemical staining:Article Title: Global epigenetic immunohistochemical markers reveal developmental connections of distinct cell types during fetal development.
Article Snippet: .. Immunohistochemical staining was performed for anti-acetyl-Histone H3 (anti-H3Ac; polyclonal rabbit (IgG) anti-human; Cat.-No: 06–599; Merck Millipore, Darmstadt, Germany; dilution 1:200), anti-acetyl-Histone H4 (anti-H4Ac; polyclonal rabbit (IgG) anti-human; Cat.-No: 06–866; Merck Millipore, Darmstadt, Germany; dilution 1:2500), anti-5-methylcytosine (anti5MeC; monoclonal mouse (IgG1) all species; clone 4111; Cat.-No: NBP2-42814; Novus Biologicals, Darmstadt, Germany; dilution 1:150) and anti-5-hydroxymethylcytosine (anti-5hMeC; monoclonal mouse (IgG2b) all species; clone 317; Cat.-No: MA5-23525; Thermo Fisher Scientific, Dreieich, Germany; dilution 1:1500). ..
Staining:Article Title: Global epigenetic immunohistochemical markers reveal developmental connections of distinct cell types during fetal development.
Article Snippet: .. Immunohistochemical staining was performed for anti-acetyl-Histone H3 (anti-H3Ac; polyclonal rabbit (IgG) anti-human; Cat.-No: 06–599; Merck Millipore, Darmstadt, Germany; dilution 1:200), anti-acetyl-Histone H4 (anti-H4Ac; polyclonal rabbit (IgG) anti-human; Cat.-No: 06–866; Merck Millipore, Darmstadt, Germany; dilution 1:2500), anti-5-methylcytosine (anti5MeC; monoclonal mouse (IgG1) all species; clone 4111; Cat.-No: NBP2-42814; Novus Biologicals, Darmstadt, Germany; dilution 1:150) and anti-5-hydroxymethylcytosine (anti-5hMeC; monoclonal mouse (IgG2b) all species; clone 317; Cat.-No: MA5-23525; Thermo Fisher Scientific, Dreieich, Germany; dilution 1:1500). ..
other:
Recombinant:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
Transfection:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
RNA sequencing:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
Gene Expression:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
Plasmid Preparation:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
Software:Article Title: TRPM3 mediates spontaneous pain and mechanical allodynia in a mouse model of chronic orofacial neuropathy.
Article Snippet: .. They were housed in standard plastic mice cages (2–5 mice per cage) in a colony room with a room temperature of 21 ± 1 ◦ C, a relative REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-SPRR1A antibody (rabbit) Novus Biologicals Cat# NBP2-93464; RRID: AB_3463407 Anti-Calbindin 2 antibody (rabbit) Abcam Cat# ab108404; RRID: AB_10861236 Anti-PGP9.5 antibody (rabbit) Dako Cat# Z5116; RRID; AB_2622233 Biotinylated pig anti-rabbit Dako Cat# E0353; RRID: AB_2737292 Anti-Rabbit Immunoglobulins/HRP (goat) Dako Cat# P0448; RRID: AB_2617138 Chemicals, peptides, and recombinant proteins Isosakuranetin PhytoLab N/A Primidone Sigma-Aldrich P7423 Carbamazepine Sigma-Aldrich C4024 Mavatrep Sanbio HY-16935-100 Tween80 Sigma-Aldrich P4780 λ-carrageenan TCI C2871 Hematoxylin & Eosin (H&E) Sigma-Aldrich HHS16 TransIT-LT1 transfection reagent Mirus Bio MIR 2306 Critical commercial assays innuPREP RNA Mini Kit 2.0 Innuscreen GmbH 845-KS-2040250 Deposited data Bulk RNAseq data of trigeminal ganglia This paper Gene Expression Omnibus (GEO) repository: GSE316925 Experimental models: Cell lines Human: HEK293T ATCC CRL-3216 Experimental models: Organisms/strains Wild type C57Bl/6J mice (10-week old) Jackson Laboratory JAX: 000664 Trpm3 − /− C57Bl/6J mice (10-week old) Vriens et al. 24 N/A Recombinant DNA Plasmid: pCAGGSM2-hTRPM3-Ires-GFP Burglen et al. 82 N/A Plasmid: pCAGGSM2-hTRPM3(A1657V)-Ires-GFP This paper N/A Software and algorithms Origin OriginLab Corporation OriginPro 2023b STAR aligner GitHub v2.7.10b RSEM GitHub v1.3.1 DESeq2 Bioconductor v1.44.0 DOSE Bioconductor v4.0.0 enrichplot Bioconductor v1.26.6 QuPath GitHub V0.5.1 PatchMasterPro HEKA Elektronik V2x92 Other Chromic catgut ligatures (6-0) Ethicon 1836G Vicryl Rapide sutures (6-0) Ethicon W9911 e1 Cell Reports Medicine 7, 102645, March 17, 2026 OPEN ACCESS humidity of 40 ± 10% and a 12h light/hour dark cycle. ..
Control:Article Title: LBR nucleoplasmic domains regulate X-chromosome solubility and nuclear organization
Article Snippet: .. To visualize endogenous LBR, control cells were permeabilized with 0.2% Triton X-100 in PBS for 10 min, blocked with 0.5% fish skin gelatin (FSG) in PBS for 30 min and then probed with a primary anti-LBR rabbit polyclonal diluted 1:1000 (Novus, NBP2-56993) and secondary antibody (Alexa 488 goat anti-rabbit diluted 1:400). .. DNA staining of both control and transfected cells was performed with 1 mg/ml propidium iodide (PI) following treatment with 0.2 u/μl RΝase for 30 min. After 3x washing, the coverslips were mounted in 90% glycerol and visualized in a Nikon confocal microscope using the EZ-C1 3.20 software.
Enzyme-linked Immunosorbent Assay:
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